RT-PCR Primer Sequences for a Cholesterol and Hypertension Study in Rats

Primer sequences used for RT-PCR in a study examining the effects of dietary cholesterol on stroke-prone hypertensive rats. The dataset is 5.5 KB in size, authored by Yutaro Nishikata, and was last updated on 2026-05-21. The associated research found a 9–10% reduction in systolic blood pressure and altered lipid profiles in rats fed a cholesterol-enriched diet.

Use Cases

• Replicate gene expression analysis based on the RT-PCR primers described in the study
• Study lipid metabolism pathways based on the described changes in phytosterol and arachidonic acid ratios
• Investigate molecular mechanisms of hypertension based on the reported suppression of the AA-PGE2 axis
• Design follow-up experiments on dietary cholesterol effects using the provided primer sequences

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse with attribution.
• Associated research provides a clear mechanistic context, linking primer use to specific physiological outcomes like a 9–10% blood pressure reduction.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 5.5 KB size indicates a very limited scope, likely containing only primer sequences.

Provenance

Source

figshare

Collection Method

Likely generated as part of a controlled laboratory experiment described in the associated research publication.

Freshness

Last updated 2026-05-21 17:27:01; freshness should be verified.