AS-qPCR Reaction Setup for Globodera pallida Virulence SNP T173N

A 15.8 KB Excel dataset uploaded to figshare by Arno S. Schaveling on May 6, 2026. The data relates to an allele-specific quantitative PCR (AS-qPCR) assay developed to estimate virulence in the potato cyst nematode Globodera pallida. The assay determines the alternative allele frequency of a specific SNP (T173N) associated with virulence against the GpaVvrn resistance gene in potato varieties.

Use Cases

• Estimating nematode virulence levels in field populations based on the T173N SNP allele frequency described in the study.
• Developing sustainable resistance management strategies for farmers based on population-specific virulence data.
• Comparing genetic adaptation of Globodera pallida populations across Western European regions as mentioned in the research.
• Validating molecular assay results against nematode reproduction rates on resistant potato varieties.

Strengths

• Dataset is openly licensed under CC-BY-4.0.
• File size is precisely known (15.8 KB), indicating a small, focused dataset.
• The description details a specific, validated molecular assay (AS-qPCR) for a key virulence SNP.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect geographic bias inherent to the studied Dutch, British, French, and German populations.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from an allele-specific quantitative PCR (AS-qPCR) assay, as described in the associated research.

Time Range

null

Freshness

Last updated 2026-05-06 17:39:13; freshness should be verified.

Geography

The study mentions populations from the Netherlands, United Kingdom, France, and Germany, suggesting a Western European focus.