Flow Cytometry Pulse Data for Particle Property Estimation

Data supporting validation of a method to estimate particle diameter, velocity, and brightness from time-resolved flow cytometry signals. The dataset was created by Megan A. Catterton and last updated on 2026-05-18. It includes results from experiments varying flow rates on microspheres and applying the method to cells labeled with a DNA binding dye.

Use Cases

• Validate models for estimating particle diameter based on time-resolved signal shape.
• Study the impact of flow velocity changes on fluorescence pulse characteristics.
• Classify cell cycle phases (G0/G1 vs G2) based on estimated cell volume from pulse data.
• Benchmark signal processing algorithms for deconvolving particle properties from raw cytometry pulses.

Strengths

• Data is derived from controlled experiments with independently varied velocity, size, and dye concentration.
• Method validation includes a reported 1.26-fold increase in apparent diameter from G0/G1 to G2 cell populations.
• Dataset is openly licensed under CC-BY-4.0.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is relatively small at 394.2 KB, indicating limited scope.

Provenance

Source

Megan A. Catterton via figshare

Collection Method

Experimental data from flow cytometry measurements on microspheres and cells under controlled flow conditions.

Freshness

Last updated 2026-05-18 17:23:04