Flow Cytometry Pulse Data for Particle Property Estimation

Experimental data supporting validation of a method to estimate particle diameter, velocity, and brightness from time-resolved flow cytometry signals. The dataset was created by Megan A. Catterton and last updated on 2026-05-18. It includes results from controlled flow rate variations on microspheres and cells labeled with a DNA binding dye.

Use Cases

• Validate particle diameter estimation methods based on time-resolved fluorescence pulse shape.
• Study the impact of particle velocity changes on signal morphology under controlled flow conditions.
• Classify cell cycle phases (G0/G1 vs. G2) based on DNA label concentration inferred from pulse data.
• Develop algorithms to deconvolve particle properties from the laser and optical system's convolution effects.

Strengths

• Data is derived from controlled experiments with independently varied particle velocity, size, and dye concentration.
• Validates a method with a reported 1.26-fold increase in apparent diameter from G0/G1 to G2 cell populations.
• Published under the open CC-BY-4.0 license on the figshare platform.

Limitations

• The dataset is small at 55.5 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Data collected through a series of flow rate variations on microspheres of known size and fluorophore concentrations, and applied to cells labeled with a DNA binding dye.

Freshness

Last updated 2026-05-18 17:23:05; freshness should be verified.