Flow Cytometry Pulse Data for Particle Property Estimation

An Excel file contains time-resolved signal data from flow cytometry experiments. The data supports validation of a method to estimate particle diameter, velocity, and brightness from pulse shapes, applied to microspheres and cells labeled with a DNA dye. The dataset was authored by Megan A. Catterton and last updated on May 18, 2026.

Use Cases

• Validating models for estimating particle diameter from time-resolved fluorescence pulses.
• Analyzing the impact of flow velocity changes on signal shape for particle characterization.
• Classifying cell cycle phases (G0/G1 vs. G2) based on inferred cell size from pulse data.

Strengths

• Data is associated with a published scientific figure (Fig. 5), indicating a research context.
• The description details a specific experimental validation involving microspheres of known size and fluorophore concentration.
• The dataset is small (22.6 KB), facilitating quick download and initial inspection.

Limitations

• The specific columns and row count are unknown, requiring inspection after download to understand the data structure.
• The dataset's scope is limited to supporting a specific figure, which may constrain its general applicability.
• Documentation is limited to the provided description; column-level semantics must be inferred.

Provenance

Source

figshare

Collection Method

Likely contains experimental measurements from flow cytometry where particle velocity, size, and dye concentration were independently varied.

Freshness

Last updated 2026-05-18 17:23:07.