Flow Cytometry Pulse Data for Particle Property Estimation

An Excel file contains time-resolved signal data from flow cytometry experiments. The data supports research on estimating particle diameter, velocity, and brightness from pulse shapes, validated using microspheres and cells labeled with a DNA binding dye. The dataset was authored by Megan A. Catterton and last updated in May 2026.

Use Cases

• Estimating particle diameter from time-resolved fluorescence pulses.
• Analyzing cell cycle phases (G0/G1 vs G2) based on DNA label signals.
• Validating models that deconvolve particle velocity from signal shape.
• Studying the impact of flow rate variations on signal magnitude.

Strengths

• Data is derived from controlled experiments varying particle velocity, size, and dye concentration.
• The method was validated, finding a 1.26-fold increase in apparent diameter from G0/G1 to G2 cell populations.
• Dataset is shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small at 304.0 KB, indicating a limited scope.

Provenance

Source

figshare

Collection Method

Data generated from flow cytometry experiments with microspheres and cells under controlled flow conditions.

Freshness

Last updated 2026-05-18 17:23:08; freshness should be verified.