Total Protein MS Dataset: Ubiquitylated Peptides in Mtb-Infected Macrophages

Over 46,000 ubiquitylated peptides were identified via mass spectrometry in Mycobacterium tuberculosis-infected macrophages. This dataset, created by Tina Truong and last updated in March 2026, includes roughly 400 peptides with CBL-dependent ubiquitylation. It reveals thousands of new ubiquitin-mediated signaling events and identifies FAF2 as a key regulator of immune signaling.

Use Cases

• Identify potential CBL substrates based on ubiquitylated peptide data.
• Analyze CBL-dependent regulation of antiviral response genes in infected macrophages.
• Perform genetic interaction analysis linking ubiquitin ligase activity to immune signaling pathways.
• Investigate the role of the FAF2 adapter protein in macrophage antibacterial capacity.

Strengths

• Contains over 46,000 identified ubiquitylated peptides, providing a broad view of signaling events.
• Includes roughly 400 peptides with specifically CBL-dependent ubiquitylation for focused analysis.
• Data is openly available under a CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is relatively small at 3.4 MB, suggesting it may be a focused subset rather than a large-scale proteomic screen.

Provenance

Source

figshare

Collection Method

Mass spectrometry analysis of Mycobacterium tuberculosis-infected macrophages.

Time Range

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Freshness

Last updated 2026-03-17 17:36:42.

Geography

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