Total RNAScope ISH Data: E. coli O157:H7 Adherence to Bovine Rectoanal Junction

Indira T. Kudva's dataset contains RNAScope in situ hybridization and related data on Shiga toxin-producing Escherichia coli O157:H7 adherence to bovine intestinal tissue. The 23.9 MB XLSX file includes results from RAJ squamous epithelial cell and organ culture assays, comparing wild-type, slp deletion mutant, and complemented bacterial strains. The dataset was last updated on 2026-05-18 and is shared under a CC-BY-4.0 license.

Use Cases

• Compare bacterial adherence patterns between wild-type and mutant strains based on the described RSE cell and RAJ-IVOC assays.
• Investigate the role of the Slp lipoprotein and pIgR receptor interaction in initial bacterial attachment as suggested by the in silico modeling.
• Analyze tissue integrity and histopathological disruptions caused by bacterial adherence using the described nuclear/cell staining and immunofluorescence data.

Strengths

• Dataset is 23.9 MB in size, indicating a substantial volume of data.
• Includes data from multiple experimental assays: RSE cell adherence, RAJ-IVOC adherence, immunofluorescence, and RNAscope ISH.
• Compares three distinct bacterial strains (wild-type, deletion mutant, complemented mutant) and a no-bacteria control.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

Indira T. Kudva via figshare.

Collection Method

Data generated from in vitro organ culture (IVOC) assays, cell culture adherence assays, immunofluorescence staining, and RNAscope in situ hybridization.

Freshness

Last updated 2026-05-18 17:42:51; freshness should be verified.