pGW217: Vector Map and Sequence for OspAST1 Viability Reporter Plasmid

97.1 KB of data files containing the vector map and annotated sequence for the OspAST1 viability reporter plasmid. This plasmid was used as a backbone to construct other OspA-expressing mScarlet-I reporter plasmids described in the associated study. The dataset was authored by Graham G. Willsey and last updated on April 21, 2026.

Use Cases

• Designing new genetic constructs based on the OspAST1 plasmid backbone mentioned in the description
• Verifying plasmid sequence integrity for the mScarlet-I reporter system described in the study
• Analyzing vector map features for cloning and genetic engineering workflows

Strengths

• Dataset is small (97.1 KB), ensuring quick download and inspection.
• Includes both a vector map and an annotated sequence file, providing complementary data types.
• Released under a permissive CC-BY-4.0 license, allowing for broad reuse.

Limitations

• Row count and column-level documentation are absent; data structure must be inferred after download.
• The description metadata is limited to the plasmid's purpose, with no details on experimental validation or source organism.

Provenance

Source

figshare

Collection Method

Likely generated as part of a molecular biology study to share plasmid constructs.

Freshness

Last updated 2026-04-21 17:52:05; freshness should be verified.