Vectors and Primers for Engineering an E. coli L-Fuculose Production Strain

Gun-Hwi Yeon provides a dataset of vectors and primers used in a metabolic engineering study. The dataset, shared on figshare, details genetic constructs engineered in Escherichia coli to produce the rare sugar l-fuculose. The final engineered strain achieved a production titer of 50.25 ± 4.30 mg/L.

Use Cases

• Designing genetic constructs for metabolic pathway redirection based on the described gene deletions and overexpressions.
• Replicating the engineered l-fuculose biosynthesis pathway in other microbial hosts.
• Benchmarking production titers of engineered strains against the reported 50.25 mg/L yield.
• Identifying primer sequences for cloning and validating genetic modifications in E. coli.

Strengths

• Dataset is directly associated with a published study detailing a 32.4-fold increase in l-fuculose production.
• The 11.6 KB size suggests a focused, manageable set of genetic part sequences.
• Available under a permissive CC-BY-4.0 license for reuse and modification.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset's scope is limited to the specific vectors and primers from a single study.

Provenance

Source

Gun-Hwi Yeon

Collection Method

Likely generated as part of a laboratory study engineering an E. coli metabolic pathway.

Freshness

Last updated 2026-05-14 17:34:29; freshness should be verified.