Bacterial Viability Fluorescence Images and Analysis from Parallelized Electrode Array

Supplemental data for a Scientific Reports paper on a rapid method for detecting antimicrobial resistance. The dataset likely contains time-lapse widefield fluorescence images and analysis results from a 2x2 array of microscopes measuring bacterial response to electrical stimuli. It was authored by Ka Kiu Lee and uploaded to figshare in May 2026.

Use Cases

• Training image segmentation models based on K-means clustered pixel data from time-lapse sequences.
• Analyzing fluorescence signal changes in bacterial clusters in response to electrical stimulation.
• Developing scalable viability assays for 96-well formats based on the described electrode array consumable.
• Benchmarking low numerical aperture (NA=0.25) optical detection systems for bacterial imaging.

Strengths

• Data supports a novel method achieving a viability readout in less than one minute after a 2-hour incubation.
• The experimental setup uses a parallelized 2x2 microscope array, suggesting replicated experimental conditions.
• Dataset is licensed CC-BY-4.0, permitting wide reuse with attribution.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The 37.3 MB size suggests a small dataset, which may limit machine learning applications.

Provenance

Source

Ka Kiu Lee, likely affiliated with the University of Exeter based on the 'Exeter Multiscope'.

Collection Method

Generated using a custom-built 2x2 array of parallelized microscopes with LED excitation and low NA optics.

Freshness

Last updated 2026-05-13 09:19:33; freshness should be verified.