Patch-Clamp Single-Cell Proteomics from Rat Prefrontal Cortex Neurons

An exploratory dataset from rat medial prefrontal cortex pyramidal neurons combines patch-clamp electrophysiology with single-cell proteomics to assess retrieval quality. The 627.9 KB XLSX file, authored by Larry Rodriguez and last updated in March 2026, contains results from an indiscriminate shotgun strategy where all patched neurons were collected. It provides a framework for interpreting how electrophysiological context and soma retrieval quality shape proteomic measurements in semi-intact circuits.

Use Cases

• Correlating proteome yield with electrophysiological parameters like capacitance, based on the finding that capacitance correlated with protein identifications.
• Investigating synaptic protein enrichment based on the preservation of neuronal spiking during relocation, which was associated with broader synaptic enrichment.
• Assessing the impact of retrieval quality on proteomic outcomes by comparing data from torn or aspirated neurons versus well-preserved ones.
• Developing frameworks for interpreting single-neuron proteomic measurements in the context of in situ physiology, as described in the study's methodology.

Strengths

• Data integrates two powerful techniques: patch-clamp electrophysiology and single-cell proteomics.
• Provides a specific framework for contextualizing outcomes with an emphasis on retrieval quality metrics like material yield and synaptic content.
• File size is 627.9 KB, making it a manageable download for initial exploration.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is described as exploratory, suggesting it may be a preliminary or proof-of-concept study.

Provenance

Source

figshare, authored by Larry Rodriguez.

Collection Method

Data collected from patch-clamp recordings and subsequent single-cell proteomic analysis of rat medial prefrontal cortex pyramidal neurons.

Freshness

Last updated 2026-03-19 10:30:19.