Mouse Intestinal Immune Cell Percentages in Necrotizing Enterocolitis

Flow cytometry data from a 2026 study comparing immune cell populations in the intestinal lamina propria of mice. The dataset contains percentages of CD4⁻RORγt⁺ ILC3s and CD4⁺RORγt⁺ Th17 cells among total CD45⁺ cells, comparing a control group to a group with induced necrotizing enterocolitis. It was authored by Junyu He and shared under a CC-BY-4.0 license.

Use Cases

• Compare immune cell subset proportions between healthy and diseased states based on the described experimental groups.
• Analyze the relationship between RORγt-expressing cell types and intestinal inflammation based on the flow cytometry targets.
• Validate statistical methods for group comparisons based on the described use of an unpaired two-tailed Student's t-test.
• Study the immunological basis of necrotizing enterocolitis based on the mouse model and cell populations described.

Strengths

• Includes 7 biological replicates per experimental group, providing a basis for statistical analysis.
• Experimental design is clearly described with two defined groups (CON and NEC) and specific detection targets.
• Data is shared under a permissive CC-BY-4.0 license, facilitating reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analyses.
• The dataset is 341.9 MB in FCS format, which may require specialized software (e.g., FlowJo) for analysis.

Provenance

Source

figshare

Collection Method

Flow cytometry performed on intestinal lamina propria samples from wild-type C57BL/6 mice.

Time Range

The study date is not specified; the dataset was last updated in 2026.

Freshness

Last updated 2026-04-16 03:33:49; freshness should be verified.

Geography

Laboratory study; geographic origin is not specified.