Mouse ILC3 Cytokine Production in Necrotizing Enterocolitis with Autophagy Knockout

Flow cytometry data quantifying IL-17A and IL-22 producing group 3 innate lymphoid cells (ILC3s) in the intestinal lamina propria of mice with necrotizing enterocolitis. The dataset compares autophagy-intact control mice (Atg5fl/fl) with mice having an ILC3-specific autophagy deficiency (RorccreAtg5fl/fl), with 6 biological replicates per group. Data was collected by Junyu He and last updated in April 2026.

Use Cases

• Compare cytokine production profiles between autophagy-intact and autophagy-deficient ILC3s based on the described experimental groups.
• Analyze the proportion of IL-17A+ and IL-22+ cells within total ILC3 and double-negative ILC3 subsets relative to total immune cells.
• Validate statistical findings from the described unpaired two-tailed Student's t-test comparing the two independent groups.
• Investigate the role of RORγt+ cell-specific autophagy in necrotizing enterocolitis pathology based on the flow cytometry detection targets.

Strengths

• Includes 6 biological replicates per experimental group, providing a basis for statistical analysis.
• Data was acquired using a CytoFLEX S flow cytometer with a specific panel of fluorochrome-conjugated antibodies, suggesting standardized measurement.
• Experimental design clearly defines two genetically modified mouse groups on a C57BL/6 background for controlled comparison.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• Data is specific to a mouse model of necrotizing enterocolitis, which may limit generalizability to human biology.

Provenance

Source

figshare, author Junyu He.

Collection Method

Flow cytometry performed on intestinal lamina propria samples from genetically modified mice subjected to experimental necrotizing enterocolitis induction.

Time Range

The experimental time range is not specified.

Freshness

Last updated 2026-04-16 02:26:17

Geography

The geographic origin of the data or mice is not specified.