In Vitro Model of Fuchs Endothelial Corneal Dystrophy with Senescence and ECM Data

Experimental data from a study investigating the role of cellular senescence in Fuchs endothelial corneal dystrophy (FECD). The dataset likely contains measurements from an in vitro human corneal endothelial cell model exposed to chronic stress, including gene expression, protein levels, and cell cycle analysis. The data was authored by Mohit Parekh and published via Harvard Dataverse in May 2026.

Use Cases

• Modeling the link between p16-mediated senescence and pathological ECM deposition based on described gene expression data.
• Analyzing cell cycle dynamics in stressed corneal endothelial cells based on flow cytometry results mentioned in the description.
• Comparing in vitro guttae formation with FECD patient specimens using morphological and ultrastructural data suggested by the study methods.

Strengths

• Includes specific quantitative results, such as a 42-fold upregulation of CDKN2A and 63% p16 positivity in treated cells.
• Data is derived from a biologically relevant model integrating environmental, hormonal, and aging factors as described.
• Findings are validated against human FECD tissue specimens, suggesting clinical relevance.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analysis.
• The dataset appears to be a single, static research output; update frequency is not indicated.

Provenance

Source

Harvard Dataverse, authored by Mohit Parekh.

Collection Method

Data generated from laboratory experiments on immortalized human corneal endothelial cells exposed to ultraviolet-A light and 4-hydroxyestradiol.

Freshness

Last updated 2026-05-15 22:12:09; freshness should be verified.