A methodological paper describes the use of permeabilized acinar cells as a tool for studying exocytosis. The author, Diana Dh from the University of Wisconsin–Madison, details techniques like cracked cells and cytolysin application to introduce membrane-impermeant agents into the cytoplasm. The dataset's specific size, format, and row count are not provided in the input.
Use Cases
- Study the direct effects of inhibitory peptides on membrane fission and fusion based on the methodology described.
- Investigate the role of cytosolic regulatory proteins in the secretory pathway using the permeabilized cell technique.
- Analyze calcium-dependent exocytosis by clamping intracellular Ca2+ concentration as mentioned in the description.
- Screen for novel pharmacological agents that modulate acinar cell function based on the described experimental approach.
Strengths
- The description provides a detailed methodological review of cell permeabilization techniques, referencing over 30 cited studies.
- The dataset is associated with an Open Access (gold) license, facilitating reuse.
Limitations
- Row count is unknown, which may limit suitability assessment.
- Column-level documentation is absent; field semantics must be inferred after download.
- Last update date is unknown; freshness unverified.
Provenance
- Source
- University of Wisconsin–Madison
- Collection Method
- The description reviews established laboratory methodologies for cell permeabilization, including the use of detergents and bacterial toxins.