Phage-Mediated Gold Nanoparticle Aggregation for Vibrio Parahaemolyticus Detection

A research dataset describes a colorimetric method for detecting the foodborne pathogen Vibrio parahaemolyticus using a specific lytic phage (VPP1) and gold nanoparticles. The method, developed by Zhi Li, enables detection within 75 minutes across a concentration range of 8.9 × 10¹ to 8.9 × 10⁸ CFU/mL, with validation in spiked lake water and crayfish samples. The dataset was last updated on 2026-05-02.

Use Cases

• Training machine learning models for pathogen concentration quantification based on UV-Vis spectrophotometer data mentioned in the description.
• Developing rapid diagnostic tools for food safety based on the described 75-minute visual detection method.
• Studying phage-host specificity for biosensor applications based on the characterized phage VPP1.
• Benchmarking new detection methods against the reported recovery rates of 81.07%–129.73% in spiked samples.

Strengths

• Method validated with specific performance metrics, including a detection range of 8.9 × 10¹ to 8.9 × 10⁸ CFU/mL.
• Demonstrated specificity with no cross-reaction to interfering or dead bacteria as stated in the description.
• Recovery rates provided for spiked environmental (81.07%–129.17%) and food samples (83.78%–129.73%).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset's primary content is a methodological description; the availability of underlying raw measurement data is unclear.

Provenance

Source

figshare, authored by Zhiwei Li.

Collection Method

Experimental research involving phage isolation, genomic sequencing, and development of a gold nanoparticle-based colorimetric assay.

Freshness

Last updated 2026-05-02 23:13:48; freshness should be verified.