Mouse Intestinal ILC3 Cytokine Response to Autophagy Modulation in NEC

7 biological replicates per group provide data on IL-17A and IL-22 production in intestinal immune cells. Flow cytometry data from a CytoFLEX S instrument measures cytokine percentages in ILC3 subsets from mice with necrotizing enterocolitis. Junyu He published this 2.9 GB dataset under CC-BY-4.0 in April 2026.

Use Cases

• Compare cytokine production between total and double-negative ILC3 subsets based on the described detection targets.
• Analyze the bidirectional effects of pharmacological autophagy modulation (3-Methyladenine vs. Rapamycin) on IL-17A and IL-22.
• Validate flow cytometry gating and analysis strategies for ILC3 subsets using the listed antibody panel.
• Benchmark cytokine profiles in a standardized mouse model of necrotizing enterocolitis.

Strengths

• Experimental design includes 3 clearly defined treatment groups with a vehicle control.
• Data includes 7 biological replicates per group, supporting statistical analysis.
• Specific statistical method (one-way ANOVA with Tukey’s post-hoc test) is documented.
• The full antibody panel used for flow cytometry staining is listed.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Experimental data generated via flow cytometry on a CytoFLEX S instrument from a controlled mouse study.

Time Range

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Freshness

Last updated 2026-04-16 03:20:59; freshness should be verified.

Geography

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