Mouse ILC3 Cytokine Response to Phosphatidylcholine in NEC, 12 Biological Replicates

Experimental flow cytometry data from a mouse model of necrotizing enterocolitis (NEC) on a C57BL/6 background. The dataset contains measurements of IL-17A and IL-22 production in intestinal lamina propria ILC3 subsets, comparing PBS vehicle control to phosphatidylcholine treatment across 12 mice. It was authored by Junyu He and last updated in April 2026.

Use Cases

• Compare cytokine production (IL-17A, IL-22) between treatment and control groups based on the described experimental design.
• Analyze the effect of phosphatidylcholine on specific ILC3 subsets (total and DN) based on the detection targets.
• Validate statistical findings using the described unpaired two-tailed t-test methodology.
• Replicate flow cytometry gating strategies based on the listed antibody panel (e.g., NKp46-FITC, RORγt-PerCP-Cy5.5).

Strengths

• Includes 6 biological replicates (individual mice) per group, totaling 12 samples.
• Experimental groups are fully matched to the described figure and statistical analysis (unpaired two-tailed t-test) is specified.
• Data is shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is 3.3 GB in size, which may require significant storage and specialized software (e.g., for FCS files) to analyze.

Provenance

Source

figshare

Collection Method

Data was acquired via flow cytometry (CytoFLEX S instrument) from a controlled mouse experiment.

Time Range

The temporal coverage of the underlying experiment is not specified.

Freshness

Last updated 2026-04-16 03:03:01.

Geography

The spatial coverage is not specified, but the animal model uses wild-type mice on a C57BL/6 background.