Phosphoproteomics of Shrimp Muscle Softening During Refrigeration

1255 differential abundance phosphopeptides (DAPPs) were identified from shrimp muscle proteins after 120 hours of chilling storage at 4°C. The dataset, created by Caihong Li and last updated in May 2026, profiles 2128 phosphorylated sites across 608 phosphoproteins to investigate texture deterioration mechanisms. It is hosted on figshare as a 1.7 MB XLSX file under a CC-BY-NC-4.0 license.

Use Cases

• Identify potential protein targets for food preservation based on phosphorylation changes in myofilament contraction pathways.
• Analyze kinase and phosphatase activity during storage based on SP and R/KxxS motif dephosphorylation patterns.
• Investigate protein-protein interaction networks related to texture deterioration, focusing on nodes like MAPK14 and myosin light chain kinase.
• Validate phosphoproteomics findings with orthogonal methods like Western blotting, as described for MAPK.

Strengths

• Contains data on 1255 differential phosphopeptides and 2128 phosphorylated sites, providing a detailed molecular snapshot.
• Links phosphorylation changes to specific biological pathways like mTOR signaling, autophagy, and energy metabolism.
• Includes verification data from Western blotting for a key protein (MAPK), supporting the proteomics findings.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• The dataset is small (1.7 MB), which may indicate a limited scope or sample size.

Provenance

Source

figshare

Collection Method

Phosphoproteomic analysis of shrimp muscle after chilling storage.

Time Range

Storage duration of 120 hours at 4°C.

Freshness

Last updated 2026-05-12 15:52:05; freshness should be verified.