Phosphorylation Changes in Legumain Knockout Mouse Kidneys and Liver

A proteomics study by Tilen Sever, last updated in May 2026, analyzes protein phosphorylation changes in kidney and liver samples from legumain knockout mice compared to wild-type counterparts. The dataset, 32.6 MB in size, contains results from mass spectrometry, Western blot, and immunohistochemistry validation. It identifies specific phosphorylation sites on the Y-box binding protein 1 (YBX1) and links them to activation of the NF-κB pathway.

Use Cases

• Identify differential protein phosphorylation sites based on mass spectrometry data mentioned in the description
• Investigate the link between legumain deficiency and inflammatory response based on NF-κB pathway activation findings
• Validate phosphorylation changes in specific tissues based on kidney and liver sample analysis
• Study the role of YBX1 protein modifications in cellular signaling based on identified serine 100 residue phosphorylation

Strengths

• Data is validated using multiple methods, including mass spectrometry, Western blot, and immunohistochemistry as stated in the description
• File size is 32.6 MB, indicating a substantial amount of processed data
• Dataset is associated with a specific biological finding: phosphorylation of YBX1 at serine 100 activating the NF-κB pathway

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Collection Method

Phosphopeptides from kidney and liver samples were enriched and analyzed using mass spectrometry, with validation by Western blot and immunohistochemistry.

Time Range

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Freshness

Last updated 2026-05-06 08:03:51; freshness should be verified

Geography

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