Phosphorylation Changes in Legumain Knockout Mouse Kidneys and Liver

A proteomics study by Tilen Sever, last updated in May 2026, analyzes protein phosphorylation changes in legumain knockout mice compared to wild-type counterparts. The dataset, 28.5 KB in size, contains results from mass spectrometry analysis of kidney and liver samples, validated with Western blot and immunohistochemistry. It identifies specific phosphorylation sites on the Y-box binding protein 1 (YBX1) and links a serine 100 site to NF-κB pathway activation.

Use Cases

• Analyze differential protein phosphorylation patterns based on mass spectrometry data mentioned in the description
• Investigate the link between legumain deficiency and inflammatory signaling based on identified NF-κB gene expression
• Validate phosphorylation site findings on YBX1 using the described Western blot and immunohistochemistry validation methods
• Study the molecular basis of kidney dysfunction and hematopoiesis alterations in a specific mouse model

Strengths

• Data is validated using multiple methods: mass spectrometry, Western blot, and immunohistochemistry
• File size is 28.5 KB, indicating a focused and manageable dataset
• Dataset is licensed under CC-BY-NC-4.0, allowing for non-commercial reuse with attribution

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Dataset is small (28.5 KB), indicating limited scope and likely a summary or processed results rather than raw spectra

Provenance

Source

figshare

Collection Method

Phosphopeptides from kidney and liver samples were enriched and analyzed using mass spectrometry.

Time Range

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Freshness

Last updated 2026-05-06 08:03:48; freshness should be verified

Geography

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