Phosphorylation Sites in Legumain Knockout Mouse Kidneys and Liver

Tilen Sever's dataset from figshare, last updated May 2026, contains mass spectrometry data on protein phosphorylation changes in legumain knockout mice. The 11.1 KB XLSX file likely includes identified phosphorylation sites, particularly on Y-box binding protein 1 (YBX1), comparing knockout and wild-type mice. Data was generated from kidney and liver samples using phosphopeptide enrichment, mass spectrometry, and validation via Western blot and immunohistochemistry.

Use Cases

• Identify differential protein phosphorylation sites based on mass spectrometry data mentioned in the description
• Investigate the link between YBX1 phosphorylation and NF-κB pathway activation described in the study
• Analyze the impact of legumain deficiency on kidney and liver signaling pathways referenced in the description
• Validate mass spectrometry findings using complementary methods like Western blot, as outlined in the methodology

Strengths

• Data is validated with multiple methods: mass spectrometry, Western blot, and immunohistochemistry as stated in the description
• Includes a specific, biologically significant finding: phosphorylation at serine 100 on YBX1 activates the NF-κB pathway
• File is small (11.1 KB) and in a widely accessible format (XLSX) for quick inspection

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Dataset is very small (11.1 KB), indicating limited scope and likely a focused subset of experimental results

Provenance

Source

figshare, author Tilen Sever

Collection Method

Phosphopeptides from kidney and liver samples were enriched and analyzed using mass spectrometry, with validation by Western blot and immunohistochemistry.

Time Range

null

Freshness

Last updated 2026-05 06 08:03:47; freshness should be verified

Geography

null