PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Phaeodactylum tricornutum

PHYCUT is a plasmid-based CRISPR/Cas9 system developed for multiplex genome editing in the marine diatom Phaeodactylum tricornutum. The dataset likely contains experimental results from editing three FucT genes to reduce protein fucosylation. It was authored by Emily E. Stuckless and last updated on April 14, 2026.

Use Cases

• Benchmark multiplex CRISPR/Cas9 editing efficiency based on the PHYCUT plasmid system
• Analyze fucosylation reduction in engineered strains based on the three edited FucT genes
• Design future genome engineering experiments in diatoms based on the demonstrated multiplex tool

Strengths

• Dataset is focused on a specific, novel CRISPR/Cas9 system (PHYCUT) for a model diatom.
• The description details a concrete application: multiplex editing of three FucT genes.

Limitations

• Dataset size is 17.8 KB, indicating a very limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Freshness

Last updated 2026-04-14 16:05:06