PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Genome Engineering

PHYCUT is a plasmid-based CRISPR/Cas9 system developed for multiplex genome editing in the marine diatom Phaeodactylum tricornutum. The dataset, created by Emily E. Stuckless and last updated on April 14, 2026, likely contains experimental results demonstrating the editing of three FucT genes to reduce protein fucosylation. The data is stored in an XLSX file with a size of 10.3 KB.

Use Cases

• Benchmarking multiplex CRISPR/Cas9 editing efficiency based on the described PHYCUT plasmid system
• Analyzing the impact of FucT gene edits on protein fucosylation levels mentioned in the description
• Developing strategies for humanizing glycosylation pathways in diatoms based on the experimental foundation provided

Strengths

• Dataset is associated with a specific, novel CRISPR/Cas9 tool (PHYCUT) described in the publication
• Data is openly available under a CC-BY-NC-4.0 license

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is very small (10.3 KB), indicating limited scope

Provenance

Source

figshare

Collection Method

Likely contains experimental data from the development and application of the PHYCUT CRISPR/Cas9 system

Freshness

Last updated 2026-04-14 16:05:10