PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Genome Engineering

Emily E. Stuckless published data on April 14, 2026, related to genome engineering in the marine diatom Phaeodactylum tricornutum. The dataset likely contains results from multiplex CRISPR/Cas9 editing experiments using the PHYCUT plasmid system. The data supports efforts to modify glycosylation pathways for algal biotechnology.

Use Cases

• Analyze multiplex CRISPR/Cas9 editing efficiency based on the PHYCUT plasmid system described
• Evaluate fucosylation reduction in engineered strains based on FucT gene editing results
• Compare protein glycosylation profiles based on data from strains with modified pathways

Strengths

• Dataset is openly licensed under CC-BY-NC-4.0
• File size is 10.4 KB, indicating a focused and manageable dataset

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Freshness

Last updated 2026-04-14 16:05:14; freshness should be verified