PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Phaeodactylum tricornutum

Emily E. Stuckless developed PHYCUT, a plasmid-based CRISPR/Cas9 system for multiplex genome editing in the marine diatom Phaeodactylum tricornutum. The dataset likely contains results from editing three FucT genes to reduce α(1,3)-linked core fucosylation, a modification relevant to biopharmaceutical production. The data was uploaded to figshare on 2026-04-14.

Use Cases

• Benchmark multiplex CRISPR/Cas9 editing efficiency based on the described PHYCUT system
• Analyze fucosylation reduction in secreted proteins based on the FucT gene editing results
• Design genetic engineering strategies for diatoms based on the scalable editing tool demonstration

Strengths

• Focuses on a specific, described genetic engineering tool (PHYCUT) for multiplex editing
• Addresses a concrete biotechnological limitation (immunogenic α(1,3)-linked core fucose)

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Freshness

Last updated 2026-04-14 16:05:18; freshness should be verified