PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Phaeodactylum tricornutum

A 7.0 MB dataset published on figshare in April 2026 by Emily E. Stuckless. It contains data related to the PHYCUT plasmid-based CRISPR/Cas9 system developed for multiplex genome editing in the marine diatom Phaeodactylum tricornutum. The dataset likely includes results from experiments targeting genes responsible for fucosylation.

Use Cases

• Optimizing multiplex CRISPR/Cas9 editing protocols based on PHYCUT system performance data
• Analyzing gene editing efficiency for the three FucT genes mentioned in the description
• Evaluating the reduction of protein fucosylation in engineered diatom strains

Strengths

• Dataset size is 7.0 MB, indicating a specific scope of experimental data
• Focuses on a novel CRISPR/Cas9 tool (PHYCUT) for multiplex editing in a key model organism

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

figshare

Collection Method

Likely contains experimental data generated during the development and application of the PHYCUT CRISPR/Cas9 system.

Freshness

Last updated 2026-04-14 16:05:08; freshness should be verified