PHYCUT: Multiplex CRISPR/Cas9 Editing Results for Diatom Glycosylation

PHYCUT is a plasmid-based CRISPR/Cas9 system developed to overcome the lack of a robust multiplex editing tool in the marine diatom Phaeodactylum tricornutum. Emily E. Stuckless published the dataset on figshare in April 2026, demonstrating multiplex editing of three FucT genes to reduce protein fucosylation. The 10.4 KB Excel file likely contains experimental results from applying this genome engineering tool.

Use Cases

• Analyze multiplex CRISPR/Cas9 editing efficiency based on experimental results
• Compare fucosylation levels in engineered strains based on FucT gene edits
• Validate plasmid-based genome engineering tools for diatoms based on PHYCUT system data

Strengths

• Dataset is associated with a novel CRISPR/Cas9 system (PHYCUT) described in the publication
• Focus on a specific biological hurdle: α(1,3)-linked core fucose immunogenicity

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Collection Method

Likely contains experimental data generated using the PHYCUT CRISPR/Cas9 system.

Freshness

Last updated 2026-04-14 16:05:17; freshness should be verified