PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Genome Engineering

PHYCUT is a plasmid-based CRISPR/Cas9 system developed for multiplex genome editing in the marine diatom Phaeodactylum tricornutum. The dataset likely contains experimental results demonstrating multiplex editing of three FucT genes to reduce protein fucosylation. It was created by Emily E. Stuckless and published on figshare in April 2026.

Use Cases

• Benchmark multiplex CRISPR/Cas9 editing efficiency in diatoms based on the PHYCUT system
• Analyze fucosylation reduction in engineered diatom strains based on FucT gene editing
• Design humanized glycosylation pathways for algal biotechnology based on the provided foundation

Strengths

• Dataset is associated with a novel CRISPR/Cas9 system (PHYCUT) designed for multiplex editing
• Focuses on a specific biological pathway (α(1,3) fucosylation) with three target genes (FucT)
• File is available in a standard spreadsheet format (XLSX)

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Dataset size is 17.7 KB, indicating a limited scope of data

Provenance

Source

figshare

Collection Method

Likely contains experimental data from the development and application of the PHYCUT CRISPR/Cas9 system.

Freshness

Last updated 2026-04-14 16:05:09; freshness should be verified