PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Genome Engineering

PHYCUT is a plasmid-based CRISPR/Cas9 system developed for the marine diatom Phaeodactylum tricornutum. The dataset likely contains results from multiplex editing experiments targeting three FucT genes to reduce protein fucosylation. Emily E. Stuckless published this data on figshare in April 2026.

Use Cases

• Analyze CRISPR/Cas9 editing efficiency based on multiplex guide RNA array processing
• Compare fucosylation levels in engineered strains based on FucT gene knockout data
• Validate plasmid-based genome engineering tools for diatoms based on experimental results

Strengths

• The dataset focuses on a specific, novel CRISPR/Cas9 system (PHYCUT) for a key synthetic biology platform.
• It targets a concrete biotechnological hurdle: reducing immunogenic α(1,3)-linked core fucose in diatom-produced proteins.
• The data is associated with a published scientific methodology for multiplex genome editing.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is 9.7 KB, indicating a very limited scope of data.

Provenance

Source

figshare

Collection Method

Likely contains experimental data from the development and application of the PHYCUT CRISPR/Cas9 system.

Freshness

Last updated 2026-04-14 16:05:13