PHYCUT: Multiplex CRISPR/Cas9 Editing Data for Diatom Genome Engineering

PHYCUT is a plasmid-based CRISPR/Cas9 system developed for the marine diatom Phaeodactylum tricornutum. The dataset likely contains experimental results demonstrating multiplex editing of three FucT genes to reduce protein fucosylation. Emily E. Stuckless published the data on figshare in April 2026.

Use Cases

• Benchmark multiplex CRISPR/Cas9 editing efficiency based on the described PHYCUT system
• Analyze fucosylation reduction in engineered strains based on the targeted FucT genes
• Develop models for humanizing algal glycosylation pathways based on the genome engineering foundation

Strengths

• The dataset is associated with a described method (PHYCUT) for scalable multiplex editing.
• The work targets a specific biological hurdle (α(1,3)-linked core fucose immunogenicity).

Limitations

• The dataset is only 21.9 KB, indicating a limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Experimental results from the development and application of the PHYCUT CRISPR/Cas9 system.

Freshness

Last updated 2026-04-14 16:05:19