Plasmid Sequences for Redox-Balance Growth-Coupled Selection Experiments

Plasmid sequences from metabolic engineering experiments by Aidan Cowan, published in 2026. The data supports a study on growth-coupled selection using a dual-feedstock strategy in Escherichia coli to evolve enzymes like HMG-CoA reductase. The dataset is 22.2 KB in size and was last updated on May 20, 2026.

Use Cases

• Benchmarking plasmid design strategies for redox-balance manipulation based on the described dual-feedstock approach.
• Training models to predict enzyme variant performance in growth-coupled selections based on sequence features.
• Analyzing genetic constructs for pathways involving acetyl-CoA or propionyl-CoA reduction mentioned in the study.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting reuse and modification.
• Specific publication context (Cowan et al. 2026) and experimental details are provided in the description.
• Small file size (22.2 KB) allows for quick download and inspection.

Limitations

• Row count and column-level documentation are unknown, limiting suitability assessment.
• The dataset's scope is limited to the specific experimental constructs described in the associated paper.

Provenance

Source

Aidan Cowan via figshare

Collection Method

Likely generated as part of metabolic engineering and directed evolution experiments.

Freshness

Last updated 2026-05-20 20:23:49