Plate-based SMART-Seq single-cell RNA-seq libraries from de-identified colorectal cancer patient primary tumor and peripheral blood specimens. The data profiles tumor-resident and circulating hybrid cells, together with tumor and immune control cells. The study includes data from 3 patients comprising 2 tumor specimens and 4 peripheral blood specimens, submitted by Melissa Wong to Harvard Dataverse in April 2026.
Use Cases
- Identify transcriptomic signatures of tumor-macrophage hybrid cells based on co-expression of epithelial and immune markers.
- Compare bona fide hybrid cells with artificial doublets to distinguish biological fusion from technical artifacts.
- Analyze differences between tumor-resident and circulating hybrid cells from matched patient samples.
Strengths
- Includes data from 3 colorectal cancer patients with matched primary tumor and peripheral blood specimens.
- Profiles distinct cell populations: hybrid cells, tumor controls, and immune controls.
- Uses FACS isolation and plate-based SMART-Seq for targeted single-cell profiling.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
Provenance
- Source
- Harvard Dataverse, submitted by Melissa Wong.
- Collection Method
- Single cells were FACS-isolated from fresh patient specimens into 96-well plates and profiled using Takara SMART-Seq mRNA Single Cell LP.
- Time Range
- null
- Freshness
- Last updated 2026-04-14 20:36:37; freshness should be verified.
- Geography
- null