Single SYTOX Orange Molecule Fluorescence Movie with Polarisation Camera

Ezra Bruggeman from the University of Cambridge created a fluorescence movie of single SYTOX Orange molecules dispersed on a cover glass. The data was collected using a Nikon Ti-U microscope equipped with a Thorlabs CS505MUP polarisation camera, with excitation from a 532 nm laser at 0.36 kW/cm^2 power density and a 100 ms exposure time. Specific dichroic and emission filters from Semrock were used during acquisition.

Use Cases

• Analyzing molecular orientation and rotational dynamics based on polarisation data.
• Developing tracking algorithms for single-molecule fluorescence microscopy.
• Calibrating or validating microscope setups using specified laser power and filter details.
• Studying photophysical properties of the SYTOX Orange dye under defined excitation conditions.

Strengths

• Data acquisition details are precisely specified, including laser wavelength (532 nm), power density (0.36 kW/cm^2), and exposure time (100 ms).
• Instrumentation is clearly documented with specific microscope (Nikon Ti-U) and camera (Thorlabs CS505MUP) models.
• Optical filters used (Semrock Di03-R532-t1 and FF01-582/64) are explicitly named, aiding experimental reproducibility.

Limitations

• Row count, file formats, and dataset size are unknown, which may limit suitability assessment.
• Column-level documentation is absent; data structure and semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Cambridge

Collection Method

Collected on a fluorescence microscope (Ti-U, Nikon) with a polarisation camera (CS505MUP, Thorlabs).