Polarisation camera PAINT movie of single Nile red dyes binding to lipid bilayer-coated si

A PAINT dataset movie captures the fluorescence of single Nile red molecules reversibly binding to a lipid bilayer on a 5 μm diameter silica microsphere. Images were recorded at 3 different focal planes using a Nikon Eclipse Ti2 microscope with a Thorlabs polarisation camera, excited by a 514 nm laser at 6.08 kW/cm² power density. The data was contributed by Ezra Bruggeman of the University of Cambridge.

Use Cases

• Analyzing single-molecule binding kinetics based on the reversible binding of Nile red dyes mentioned in the description
• Developing super-resolution reconstruction algorithms based on the time-series fluorescence movie data
• Studying membrane dynamics and cholesterol effects based on the DPPC + 40% cholesterol lipid bilayer composition
• Calibrating polarisation-sensitive microscopy setups based on the described camera and filter configuration

Strengths

• Data includes images recorded at three distinct focal planes (z1, z2, z3), providing depth information
• Experimental parameters are precisely documented, including laser wavelength (514 nm), power density (6.08 kW/cm²), and exposure time (50 ms)
• Specific materials are named, such as the silica microsphere (44054-5ML-F, Sigma-Aldrich) and filter models

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count and dataset size are unknown, which may limit suitability assessment
• Last update date is unknown; freshness unverified

Provenance

Source

University of Cambridge

Collection Method

Recorded on a fluorescence microscope (Eclipse Ti2, Nikon) with a polarisation camera (CS505MUP, Thorlabs).