Bacterial Community and PAH Attenuation Data from Oil Sludge Composting Microcosms

Processed 16S rRNA sequencing and physicochemical data from a 300-day laboratory composting experiment. The dataset includes 125,972 high-quality sequencing reads resolved into 359 genera across five manure-amended treatments and control. It was generated by Onyedikachi Ubani and supports a manuscript submitted to Data in Brief.

Use Cases

• Compare bacterial community diversity across different manure amendments based on 13 alpha diversity indices.
• Predict functional metabolic pathways for hydrocarbon degradation based on PICRUSt2-predicted MetaCyc and KEGG Ortholog tables.
• Analyze polycyclic aromatic hydrocarbon (PAH) removal efficiency over time based on compound-specific concentration and percentage removal data.
• Investigate correlations between physicochemical properties of amendments and microbial community structure based on TOC, TON, and TOP data.

Strengths

• Includes 125,972 high-quality sequencing reads with taxonomic assignment spanning 17 phyla and 359 genera.
• Covers 18 specific PAH compounds with initial and residual concentrations quantified over 300 days.
• Provides 13 calculated alpha diversity indices and multivariate statistical outputs (PERMANOVA, ANOSIM) for community analysis.
• Sequencing coverage exceeded 98% in all samples, indicating good depth.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a controlled laboratory microcosm study, which may not fully represent field-scale conditions.

Provenance

Source

NCBI BioProject accession PRJNA794053.

Collection Method

Laboratory co-composting microcosms incubated for 300 days. Bacterial community profiled via Illumina MiSeq sequencing of 16S rRNA V1-V3 regions.

Time Range

Experimental incubation period of 300 days.

Freshness

Last updated 2026-06-01 08:34:10; freshness should be verified.

Geography

Crude oil sludge sourced from Durban, South Africa; animal manures from Pretoria, South Africa.