OspF Substrate Profiling Data for Shigella Virulence

A dataset profiling the substrate selectivity of the Shigella flexneri virulence factor OspF, a phosphothreonine lyase. The data was generated by Nicholas P. McCurtin using in vitro assays and chemoproteomic profiling, and was last updated on April 22, 2026. The dataset is 510.7 KB in size and is stored in an XLSX file.

Use Cases

• Identifying novel OspF protein targets beyond MAPKs based on the chemoproteomic profiling described.
• Analyzing enzyme selectivity within the MAPK family based on the in vitro assay results.
• Developing new chemical or synthetic biology tools for site-specific protein editing based on the potential utility of phospholyases highlighted.

Strengths

• Data is derived from multiple experimental methods, including in vitro assays and bottom-up chemoproteomic profiling.
• The dataset includes validated new OspF targets such as Rab1A and casein kinase 2β.
• File size is 510.7 KB, making it quick to download and inspect.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's small size (510.7 KB) suggests a limited scope of data.

Provenance

Source

figshare

Collection Method

Combination of in vitro assays with synthetic phosphopeptides and bottom-up chemoproteomic profiling.

Time Range

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Freshness

Last updated 2026-04-22 19:38:20; freshness should be verified.

Geography

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