Profiling Selectivity for the Shigella Virulence Factor OspF

Nicholas P. McCurtin published this dataset on figshare in 2026. It contains results from in vitro assays and chemoproteomic profiling exploring the substrate profile of the bacterial virulence factor OspF, a phosphothreonine lyase secreted during Shigella flexneri infection. The data likely includes identified protein targets beyond the MAPK family, such as Rab1A and casein kinase 2β.

Use Cases

• Analyzing substrate selectivity within the MAPK family based on in vitro assays with synthetic phosphopeptides.
• Identifying novel cellular targets of OspF during infection based on bottom-up chemoproteomic profiling data.
• Validating new OspF targets like Rab1A and casein kinase 2β using nucleophilic phosphine chemical probe data.
• Investigating the role of phosphothreonine lyases in silencing host immune responses based on the described mechanisms.

Strengths

• Data is derived from a combination of in vitro assays and chemoproteomic profiling, suggesting a multi-method validation approach.
• The dataset identifies OspF targets beyond the previously known MAPK family, expanding the known substrate profile.
• File is provided in the structured XLSX format, which is 27.3 KB in size.

Limitations

• Row count and column-level documentation are unknown; field semantics must be inferred after download.
• The dataset's small size (27.3 KB) indicates a limited scope, likely focused on a specific experimental result set.

Provenance

Source

figshare

Collection Method

Combination of in vitro assays with synthetic phosphopeptides and bottom-up chemoproteomic profiling.

Time Range

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Freshness

Last updated 2026-04-22 19:38:21; freshness should be verified.

Geography

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