Mouse ILC3 Subset Proportions Under Autophagy Modulation in Necrotizing Enterocolitis

Junyu He published this dataset on figshare in April 2026. It contains flow cytometry data measuring the proportion of group 3 innate lymphoid cell (ILC3) subsets in the intestinal lamina propria of mice with necrotizing enterocolitis (NEC). The data includes results from 6 biological replicates per group across three experimental conditions: vehicle control, autophagy inhibition, and autophagy activation.

Use Cases

• Compare the effects of pharmacological autophagy inhibition versus activation on ILC3 populations based on the described experimental groups.
• Analyze the relationship between total ILC3s and double-negative (DN) ILC3 subsets among total innate lymphoid cells based on the detection targets.
• Validate statistical findings from the described one-way ANOVA with Tukey's post-hoc test using the underlying flow cytometry data.
• Investigate cell surface and intranuclear marker expression patterns based on the listed fluorochrome-conjugated antibodies.

Strengths

• Includes 6 biological replicates per experimental group, providing a basis for statistical analysis.
• Experimental design is clearly described with three distinct treatment groups (DMSO, 3MA, Rapamycin).
• Data was acquired using a specific instrument (CytoFLEX S flow cytometer) and a defined panel of antibodies.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical needs.

Provenance

Source

Junyu He via figshare

Collection Method

Experimental data from flow cytometry performed on wild-type C57BL/6 mice subjected to standardized NEC induction.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-16 02:37:03; freshness should be verified.

Geography

Laboratory study; geographic origin not specified.