Proteomic and Metabolomic Analysis of PCK2 in Group 3 Medulloblastoma Cells

A 1.2 MB dataset from figshare, last updated May 6, 2026, containing proteomic and metabolomic analysis results. The data was generated by Medha Gayathri J Pai to investigate the functional role of mitochondrial phosphoenolpyruvate carboxykinase (PCK2) in Group 3 medulloblastoma cells. It includes findings from shRNA-mediated knockdown experiments in HD-MB03 and G3MB cells, revealing dysregulation of metabolic interactors and pathways.

Use Cases

• Identify potential therapeutic vulnerabilities in Group 3 medulloblastoma based on PCK2-dependent metabolic alterations.
• Analyze dysregulation of ribosomal and RNA processing pathways in response to PCK2 knockdown.
• Investigate changes in metabolites like phosphocholine and carnitine linked to redox imbalance upon PCK2 loss.
• Study the link between the TCA cycle and pyruvate metabolism in cancer cell metabolic flexibility.

Strengths

• Data is associated with a specific, peer-reviewed research context investigating PCK2 in a defined cancer subtype.
• The dataset file is 1.2 MB in size, making it manageable for download and initial analysis.
• Proteomic and metabolomic data are complementary, providing a multi-omics perspective on the biological question.

Limitations

• Row count is unknown, which may limit suitability assessment for large-scale statistical modeling.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is focused on two specific cell lines (HD-MB03 and G3MB), which may limit generalizability.

Provenance

Source

figshare

Collection Method

Generated via quantitative proteomics (Evosep-Tims TOF) and complementary metabolomic profiling following shRNA-mediated knockdown experiments.

Freshness

Last updated 2026-05-06 05:14:40; freshness should be verified.