Proteomic and Metabolomic Analysis of PCK2 in Group 3 Medulloblastoma Cells

A proteomic and metabolomic dataset from a study of Group 3 medulloblastoma, an aggressive pediatric brain tumor subtype accounting for 20–25% of cases. The data was generated by Medha Gayathri J Pai and last updated on 2026-05-06, investigating the functional role of the mitochondrial enzyme PCK2 via shRNA knockdown in HD-MB03 and G3MB cells. The 9.9 KB XLSX file contains results from quantitative proteomics and complementary metabolomic profiling.

Use Cases

• Identify metabolic pathway dysregulations based on proteomic data showing enrichment of ribosomal and RNA processing pathways.
• Analyze redox imbalance and metabolic alterations based on metabolomic profiling showing changes in phosphocholine and carnitine levels.
• Investigate the role of PCK2 in metabolic flexibility under nutrient stress based on the described link between the TCA cycle and pyruvate metabolism.
• Validate potential therapeutic targets in Group 3 medulloblastoma based on the dataset's exposure of vulnerabilities from PCK2 loss.

Strengths

• Data is derived from a specific, biologically relevant model using shRNA-mediated knockdown in two cell lines (HD-MB03 and G3MB).
• Analysis integrates two complementary omics layers: quantitative proteomics and metabolomic profiling.
• Findings are contextualized with prior observations of PCK2 overexpression in two independent patient datasets.

Limitations

• Row count is unknown, which may limit suitability assessment for statistical analysis.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 9.9 KB, indicating limited scope, likely containing summary results rather than raw experimental data.

Provenance

Source

figshare, author Medha Gayathri J Pai.

Collection Method

Generated via shRNA-mediated knockdown experiments followed by quantitative proteomics (Evosep-Tims TOF) and metabolomic profiling.

Freshness

Last updated 2026-05-06 05:14:38; freshness should be verified.