Proteomic Identification of Ribosome Assembly Factors in Trypanosoma brucei

373.3 KB of proteomic data from a study investigating small-subunit ribosome assembly in Trypanosoma brucei. The dataset, authored by Gustavo Guadagnini Perez and last updated on 2026-05-18, was generated by affinity purification and mass spectrometry of tagged proteins UTP6 and PNO1. It identifies conserved and trypanosomatid-specific candidate assembly factors.

Use Cases

• Identifying trypanosomatid-specific proteins involved in ribosome biogenesis based on the described candidate factors.
• Analyzing protein-protein interaction networks in early SSU processome intermediates based on affinity purification data.
• Predicting functional roles for uncharacterized proteins based on described RNA-binding motifs and protein-protein interaction domains.
• Comparing ribosome assembly pathways between kinetoplastids and other eukaryotes based on the identified conserved and unique factors.

Strengths

• Data is derived from a controlled experimental method using affinity purification and mass spectrometry.
• The study provides complementary access to different ribosome assembly stages via tagged versions of UTP6 and PNO1.
• Dataset includes a substantial set of trypanosomatid-specific proteins with no previously assigned function.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (373.3 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Affinity purification followed by mass spectrometry of tagged proteins in Trypanosoma brucei cell lines.

Freshness

Last updated 2026-05-18 20:08:57; freshness should be verified.