Trypanosoma brucei Pre-Small-Subunit Ribosome Assembly Proteomic Data

2.9 MB of proteomic data from affinity purification and mass spectrometry analysis of pre-small-subunit ribosome complexes in Trypanosoma brucei. The dataset, published by Gustavo Guadagnini Perez on figshare in 2026, identifies conserved ribosome biogenesis factors and trypanosomatid-specific proteins. It provides a foundation for functional studies of species-specific ribosome assembly mechanisms.

Use Cases

• Identify candidate ribosome assembly factors based on affinity purification data from tagged UTP6 and PNO1 proteins.
• Analyze species-specific adaptations in ribosome biogenesis based on the set of trypanosomatid-specific proteins with no assigned function.
• Predict protein function based on structural analyses of RNA-binding motifs and protein-protein interaction domains mentioned in the description.
• Compare ribosome assembly pathways between trypanosomatids and other eukaryotes using the identified conserved ribosome biogenesis factors.

Strengths

• Data is derived from complementary access to early and late pre-40S maturation stages via tagged UTP6 and PNO1.
• Identifies a substantial set of trypanosomatid-specific proteins alongside conserved factors.
• File is provided in the structured XLSX format, facilitating analysis.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Affinity purification followed by mass spectrometry of tagged proteins in Trypanosoma brucei cell lines.

Freshness

Last updated 2026-05-18 20:08:58; freshness should be verified.