Proteomic Data on Nepetin's Antioxidant Effects in ARPE-19 Cells

Xi Chen's dataset contains quantitative proteomics results from a study on the flavonoid nepetin's protective effects against oxidative stress in ARPE-19 retinal pigment epithelial cells. The data, shared on figshare, includes lists of 77 significantly up-regulated and 198 down-regulated proteins, with supporting bioinformatics analyses. The dataset was last updated on 2026-05-07.

Use Cases

• Identify protein expression changes linked to oxidative stress protection based on the 275 significantly altered proteins.
• Analyze pathways involved in antioxidant responses based on the mentioned key proteins HO-1, KEAP1, p62, and GRP78.
• Investigate the role of Nrf2 signaling in flavonoid-mediated cytoprotection based on the described nuclear translocation evidence.
• Validate proteomic findings from in vitro models of retinal degeneration using the provided Western blot and immunofluorescence correlates.

Strengths

• Quantitative proteomics provides specific counts of 77 up-regulated and 198 down-regulated proteins.
• Bioinformatic validation includes Gene Ontology and protein-protein interaction network analyses.
• Key protein alterations (HO-1, p62, GRP78 up, KEAP1 down) are confirmed by Western blotting.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The study describes correlative evidence; direct causal interactions were not established.

Provenance

Source

figshare, author Xi Chen

Collection Method

Experimental proteomics and bioinformatics analysis from a cell culture study.

Freshness

Last updated 2026-05-07 17:58:39; freshness should be verified.