Ram Sperm Proteomic Profiles Under Astaxanthin and Melatonin Cryoprotection

182 differentially abundant proteins were identified across three treatment comparisons of cryopreserved ram sperm. The dataset contains proteomic profiles from an Orbitrap Astral DIA analysis, comparing sperm treated with astaxanthin, melatonin, or a combination against a control. Author Chunyan Li uploaded the data to figshare in May 2026.

Use Cases

• Identify potential protein biomarkers for sperm quality based on the list of positive and negative proteins like ACAT2 and Cyt c.
• Analyze synergistic effects of antioxidants on sperm proteomes using the 116 DAPs from the combined treatment group.
• Study pathways related to oxidative stress and sperm motility enriched from the differentially abundant proteins.
• Compare individual versus combined cryoprotectant efficacy using the three defined comparisons (AST vs CON, MLT vs CON, AST.MLT vs CON).

Strengths

• Proteomic data is derived from a defined experimental design with specific treatment concentrations (50 μM AST, 0.5 mM MLT).
• Includes a substantial number of differentially abundant proteins for analysis (32, 34, and 116 across comparisons).
• Raw data is available in an accessible XLSX format with a file size of 3.9 MB.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect experimental bias inherent to the specific study design on figshare.

Provenance

Source

figshare

Collection Method

Experimental proteomic profiling using an Orbitrap Astral with data-independent acquisition (DIA).

Freshness

Last updated 2026-05-08 15:08:27; freshness should be verified.