Proteomics: Mass Spectrometry Data from CRC Cells with MKKS3 Expression

Proteomics data from colorectal cancer (CRC) cells expressing MKKS3 and control cells, processed via trypsin digestion and analyzed using an Orbitrap Fusion Lumos mass spectrometer. The dataset is 7.1 GB in size, authored by Hui-Fang Hu, and was last updated on April 22, 2026. Raw data were processed with Proteome Discoverer and Spectronaut software, with a protein/peptide false discovery rate set at 1%.

Use Cases

• Identify differentially expressed proteins based on mass spectrometry peptide analysis.
• Benchmark proteomics data processing pipelines using the described 1% FDR threshold.
• Study protein profiles associated with MKKS3 expression in colorectal cancer cells.

Strengths

• Data processed with a strict 1% false discovery rate for proteins/peptides.
• Generated using high-resolution Orbitrap Fusion Lumos mass spectrometry.
• Dataset size of 7.1 GB suggests substantial raw data volume.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analyses.

Provenance

Source

Hui-Fang Hu via figshare

Collection Method

Proteins from lysed CRC cells were digested with trypsin, desalted, and analyzed by mass spectrometry.

Time Range

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Freshness

Last updated 2026-04-22 15:11:32; freshness should be verified.

Geography

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