Arabidopsis Seedling Proteomics and Transcriptomics in Microgravity

National Aeronautics and Space Administration conducted an experiment where Arabidopsis seeds germinated and grew for 3 days on the International Space Station under microgravity conditions. The study produced proteomic and transcriptomic data from seedlings fixed with RNAlater, with ground controls used for comparison. Data was last updated on 2026-03-13.

Use Cases

• Identify differentially expressed proteins in microgravity based on iTRAQ-labeled mass spectrometry data
• Compare gene expression patterns between spaceflight and ground control seedlings
• Study the role of auxin distribution in gravitropism using molecular profiling data
• Analyze the effects of a 3-day microgravity exposure on early seedling development

Strengths

• Experimental design includes ground controls for comparison with spaceflight samples
• Sample processing involved pooling tissue from five plates to create three biological replicates
• Proteins were extracted from both membrane and soluble fractions

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Description metadata is limited; actual data quality requires manual inspection after download

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Arabidopsis seeds were flown to the International Space Station, germinated and grown for 3 days in microgravity, then fixed with RNAlater. Proteins were extracted, trypsin digested, labeled with iTRAQ, and identified using tandem mass spectrometry.

Freshness

Last updated 2026-03-13 20:12:36.866697; freshness should be verified

Geography

International Space Station