Proteotoxic Stress Bioreporter Data for Mechanism-Informed Antibiotic Discovery

A 21.3 KB Excel dataset from figshare details a novel bioreporter for antibiotic discovery. Julian Schubert developed the P_clpE-lux bioreporter to signal proteotoxic stress in Bacillus subtilis, enabling high-throughput screening. The dataset, last updated in May 2026, consolidates validation results and identifies antibacterial agents from natural product extracts.

Use Cases

• Validate and benchmark new antibacterial compounds based on the bioreporter's response to proteotoxic stress.
• Screen complex natural product extracts for novel antibiotics based on the described microfractionation-metabolomics workflow.
• Identify antibacterial agents not previously associated with proteotoxic stress based on the bioreporter's mechanism-informed detection.
• Develop high-throughput screening assays for proteotoxic stress in both solid and liquid formats based on the self-sustained luminescence feature.

Strengths

• Dataset is validated with reference antibiotics, as stated in the description.
• The bioreporter enables monitoring in both solid and liquid assay formats, supporting versatile screening.
• File size is precisely known at 21.3 KB, indicating a focused and manageable dataset.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset's 21.3 KB size indicates a very limited scope, likely containing summary or validation results rather than raw screening data.

Provenance

Source

figshare, authored by Julian Schubert.

Collection Method

Likely contains experimental results from the validation and application of the P_clpE-lux bioreporter.

Time Range

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Freshness

Last updated 2026-05-15 11:20:21; freshness should be verified.

Geography

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