LRP2-APEX2 Mouse Model: Protein Interactions in Kidney Endocytosis

LRP2-APEX2-expressing mice were engineered to biotinylate cytoplasmic partners of the Lrp2 receptor in vivo. The resulting dataset likely contains mass spectrometric identifications of proteins interacting with Lrp2, revealing its role in apical membrane organization. The data was published by Tian H. Shen on figshare in April 2026.

Use Cases

• Identify novel protein-protein interactions based on the biotinylation technique described.
• Analyze the role of PDZ domain proteins in apical membrane organization based on the findings mentioned.
• Compare endocytic regulators and molecular motors associated with Lrp2 based on the identified interactions.
• Study the functional consequences of Lrp2 deletion on protein interactions based on the described abolition of interactions.

Strengths

• Dataset is derived from a specific engineered mouse model (LRP2-APEX2-expressing mice), providing a controlled experimental system.
• The biotinylation technique was validated by both mass spectrometry and immunostaining, as stated in the description.
• File is provided in XLSX format (26.5 KB), which is readily accessible for analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset's 26.5 KB size suggests a limited scope, likely containing a focused list of interactions rather than extensive raw data.

Provenance

Source

figshare

Collection Method

In vivo biotinylation and mass spectrometric identification from kidney lysate of engineered mice.

Freshness

Last updated 2026-04-20 18:08:57; freshness should be verified.